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Influence of electrostatic interactions and enzyme reactivity on the binding mode of alpha-amylase

✍ Scribed by Engin Çörüşlü; Burhan Peki̇n

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 434 KB
Volume: 127
Category: Article
ISSN: 0008-6215
DOI: 10.1016/0008-6215(84)85363-x

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✦ Synopsis

Alpha-amylase was coupled to cellulose and carboxymethylcellulose through activation with cyanogen bromide. The specific activities, retentions of native enzyme activity, and action patterns of the resulting immobilised derivatives depended markedly on the pH of the coupling reaction. This dependency is discussed in terms of the role of the coupling pH in the regulation of electrostatic interactions and enzyme reactivity.

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