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Influence of DNA extraction and PCR amplification on studies of soil fungal communities based on amplicon sequencing

✍ Scribed by Xu, Lihui; Ravnskov, Sabine; Larsen, John; Nicolaisen, Mogens

Book ID
120172622
Publisher
NRC Research Press
Year
2011
Tongue
French
Weight
136 KB
Volume
57
Category
Article
ISSN
0008-4166

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Spectroscopic and calorimetric melting studies of 28 DNA hairpins were performed. These hairpins form by intramolecular folding of 16 base self-complementary DNA oligomer sequences. Sequence design dictated that the hairpin structures have a six base pair duplex linked by a four base loop and that t