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Influence of days of culture on cryoprotectant-supplemented medium and of terminal freezing temperature on the survival of cryopreserved pea shoot tips

โœ Scribed by Sean Mcadams; Doraiswamy Ratnasabapathi; Robert A. Smith


Book ID
115851296
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
480 KB
Volume
28
Category
Article
ISSN
0011-2240

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Reflexed saltmarsh-grass suspension cultures produced by seed callus were frozen to the liquid nitrogen temperature. Cooling rates, cryoprotectants and holding times were taken as a function of transfer temperatures. The highest survival of cells (45%) was found at a freezing rate of IยฐC rain -I , w