Influence of culture conditions on the production of heterologous interleukin 1β byKluyveromyces lactis

✍ Scribed by Blondeau Karine; O. Boutur; Boze HeTene; G. Moulin; P. Galzy

• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 454 KB
• Volume: 7
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00156338

✦ Synopsis

Sl~nmw: Under the control of the repressible PHOS promoter, the expression of gene encoding interleukin Ill (1116) was derepressed when the medium was depleted of free inorganic phosphate (Pi). Maximum heterologous protein synthesis was obtained in the presence of 75 mg KH2P04/1 (for 20 g glucose/l). The successful heterologous protein production greatly depends on nutritional culture conditions as Ill B production effwiency was increased by 83% through optimization of the growth medium. Comparison of diieient phosphate-limited cultivation strategies led to the development of a batch culture procedure with nutrient pulses to delay induced oxido-fermentative glucose metabolism and increase the 1116 production to 135 mg/l.