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Influence of colchicine treatment on chromosome number and growth rate of tissue cultures ofValeriana wallichiiDC

✍ Scribed by Supa Chavadej; Hans Becker


Book ID
104618649
Publisher
Springer
Year
1984
Tongue
English
Weight
463 KB
Volume
3
Category
Article
ISSN
0167-6857

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✦ Synopsis


Cell cultures of Valeriana wallichii were treated with 0.05%, 0.2% and 0.5% of colchicine. The treatment with 0.05% and 0.2% colchicine resulted in well growing cultures. At the highest dose the cells died. The colchicine treatment could be repeated after six alkaloid free passages. The chromosome numbers shifted to polyploidy (n > 96) under the treatment but had a strong tendency to the initial pattern.