Inflammatory cytokines up-regulate intercellular adhesion molecule-1 expression on primary cultured mouse hepatocytes and T-lymphocyte adhesion
✍ Scribed by Masahiko Morita; Yoshifumi Watanabe; Toshihiro Akaike
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 633 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0270-9139
No coin nor oath required. For personal study only.
✦ Synopsis
We investigated the effect of inflammatory cytokines on the intercellular adhesion molecule-1 expression on primary cultured murine hepatocytes. Tumor necrosis factor-a, interferon-y and interleukin-la up-regulated the intercellular adhesion molecule-1 expression on hepatocytes in a dose-dependent fashion; however, interleukin-6 did not. On the basis of kinetic analysis, the expression level reached a peak 24 hr after stimulation, and both cycloheximide and actinomycin D inhibited the expression. Furthermore, T lymphocytes bind more to interferon-y-stimulated hepatocytes than to unstimulated hepatocytes. The binding was dependent on the concentration of interferon-y. The