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Infection of human amnion cells with cytomegalovirus

โœ Scribed by Manuel E. Figueroa; Laszlo Geder; Fred Rapp

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
955 KB
Volume
2
Category
Article
ISSN
0146-6615

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โœฆ Synopsis

Abstract

Human cytomegalovirus (CMV), known to replicate in vitro in human fibroblastic cells, was found to replicate in epithelial human amnion (HA) cells. Large syncytia formed in these cells after infection with CMV; inclusion bodies were observed in the nuclei, and CMV antigens were demonstrated in both the cytoplasm and the nucleus by indirect immunofluorescence techniques. The synthesis of virus DNA was also detected, and the production of infectious virus was followed. The titers were lower (from 10^4^ to 6 ร— 10^5^ using different isolates of CMV) than those obtained in human embryo fibroblast (HEF) cells, and the replication cycle was slower in HA than in HEF cells.

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