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Infection of epithelial cell line HEp-2 with human immunodeficiency virus type 1 is CD4 dependent

✍ Scribed by Hans S. L. M. Nottet; Ingmar Janse; Loek De Graaf; Leendert J. Bakker; Maarten R. Visser; Jan Verhoef

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
661 KB
Volume
40
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

HEp‐2 cells, human epithelial cells derived from a larynx carcinoma, were found to be highly susceptible to infection with HIV‐1 stain Illb and MN, but not to infection with the monotropic strain IllBa‐L or the clinical isolate HIV‐1~AT~. HEp‐2 cells infected with HIV‐1~IIIb~ continuously secreted high levels of p24 antigen, while no cytopathic effects were observed. Although no CD4 antigen could be detected on the cells by flow cytometric analysis, CD4 mRNA was detected by reverse transcriptase PCR. Furthermore, infection could be blocked by anti‐CD4 monoclonal antibody OKT4a indicating a CD4 mediated viral entry in HEp‐2 cells. HEp‐2 cells are commonly used in clinical virology for the culture of different viruses from clinical specimens. HEp‐2 cells should therefore be handled with caution as they may potentially become infected with HIV. © 1993 Wiley‐Liss, Inc.

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