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Inefficient expression of the DNA-binding domain of GAL4 in transgenic plants

✍ Scribed by Christoph Reichel; Dorothee Feltkamp; Richard Walden; Hans-Henning Steinbiß; Jeff Schell; Sabine Rosahl

Publisher
Springer
Year
1995
Tongue
English
Weight
552 KB
Volume
14
Category
Article
ISSN
0721-7714

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✦ Synopsis

The DNA-binding domain of the yeast transcriptional activator GAL4 was expressed in transgenic tobacco plants in order to attempt to obtain specific repression of reporter genes. Expression of the GUS and the NPTII gene was essentially the same regardless of the presence of a gene encoding a functional GAL4 DNA-binding domain or the presence of GAL4 recognition sequences in the promoter region of the reporter genes. Despite high levels of GAL4 mRNA, no translation product was detectable in transgenic plants indicating that the failure to detect functional GAL4 in plants is due to the inefficiency of GAL4 mRNA translation in plants.

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Effects of DNA binding and metal substit
Effects of DNA binding and metal substitution on the dynamics of the GAL4 DNA-binding domain as studied by amide proton exchange
✍ Ted Mau; James D. Baleja; Gerhard Wagner 📂 Article 📅 1992 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 955 KB

## Abstract Backbone amide proton exchange rates in the DNA‐binding domain of GAL4 have been determined using ^1^H–^15^N heteronuclear correlation NMR spectroscopy. Three forms of the protein were studied — the native Zn‐containing protein, the Cd‐substituted protein, and a Zn‐GAL4/DNA complex. Exc