𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Inductive effect of recombinant human interleukin-1 α and β on differentiation of macrophage-like tumor cell line P388D1

✍ Scribed by Shigemasa Hanazawa; Chika Hanaizumi; Shigeru Amano; Kimiharu Hirose; Yoshihiro Ohmori; Masayoshi Kumegawa; Kohichi Yamaura; Shigeo Kitano

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
434 KB
Volume
136
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

We used the mouse monocyteimacrophage-like tumor cell line P388D1 to test whether or not interleukin-1 (IL-1) stimulates differentiation of monocyte/ macrophage progenitors. Incubation of these cells with recombinant human interleukin-1 (rhlL-1) a and p resulted in their increased adherence, stimulation of nonspecific esterase activity, and increased Fc rosette formation. rhlL-1 s inhibited cell growth and stimulated Fc rosette formation in a dose-dependent fashion. The cell growth inhibition due to rhlL-1s depended on the concentration of serum in culture medium. Synergism between rhlL-1 and calcium ionophore A231 87 was found for the cell growth inhibition and Fc rosette formation. The presence of ethylene glycol bis-(P-aminoethyl ether) N,N,N,N-tetraacetic acid(EGTA) in the medium abolished the stimulatory effect of rhlL-1 on Fc rosette formation of the cell line. These results demonstrate that rhlL-1s are a potent inducer of the differentiation of the macrophage-like tumor cell fine P388D1.

📜 SIMILAR VOLUMES

Induction of human immunodeficiency viru
Induction of human immunodeficiency virus type 1 replication in human glial cells after proinflammatory cytokines stimulation: Effect of IFNγ, IL1β, and TNFα on differentiation and chemokine production in glial cells
✍ Nazila Janabi; Mariantonietta Di Stefano; Christine Wallon; Christiane Hery; Fra 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 450 KB 👁 1 views

Although evidence for human immunodeficiency virus 1 (HIV-1) presence in the central nervous system (CNS) of infected patients is well established, the intensity of viral replication within the brain is not usually known. In vitro, human embryonic microglial cells internalized HIV-1 through a CD4-de

Contribution of tumor necrosis factor α
Contribution of tumor necrosis factor α and interleukin-1 α on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in a murine macrophage cell line, RAW264.7
✍ Sakai, Shinya; Ochiai, Hiroshi; Kawamata, Hiroshi; Kogure, Toshiaki; Shimada, Yu 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 72 KB 👁 1 views

The production of several inflammatory cytokines, such as murine macrophage inflammatory protein-2 (MIP-2), tumor necrosis factor (TNF), and interleukin (IL)-1, was investigated in response to respiratory syncytial virus (RSV) infection in a murine macrophage cell line, RAW264.7, with special refere