๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Induction of triploid and gynogenetic diploid axolotls (Ambystoma mexicanum) by hydrostatic pressure

โœ Scribed by Gillespie, Laura L. ;Armstrong, John B.

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
369 KB
Volume
210
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

Hydrostatic pressure of 10,000 psi, applied 60 minutes from oviposition, for eight minutes, was used to suppress the formation of the second polar body in fertilized axolotl eggs. When this procedure was used on naturally fertilized eggs, triploid axolotls were produced with 97% efficiency. Artificial insemination with ultraviolet irradiation inactivated sperm in combination with this procedure yielded gynogenetic diploids with 92% efficiency. Survival was variable in both sets of experiments and ranged from 64% to 72%.

๐Ÿ“œ SIMILAR VOLUMES

Suppression of first cleavage in the mex
Suppression of first cleavage in the mexican axolotl (Ambystoma mexicanum) by heat shock or hydrostatic pressure
โœ Gillespie, Laura L. ;Armstrong, John B. ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 423 KB

## Abstract Androgenetic diploid axolotls were produced by ultraviolet inactivation of the egg pronucleus shortly after fertilization, followed by suppression of the first cleavage division by hydrostatic pressure or heat shock. After treatment at 14,000 psi for 8 minutes, diploidy was restored in