Induction of Tolerance to Freeze-Thaw (FT) Damage in Mammalian Cells by Pre-FT Hypothermia Treatment

Induction of tolerance to hypothermia an

Induction of tolerance to hypothermia and hyperthermia by a common mechanism in mammalian cells

✍ David J. Glofcheski; Michael J. Borrelli; Diane M. Stafford; Jack Kruuv 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 862 KB

## Abstract Pretreatment by hypothermic (25°C) cycling (PHC) of attached exponential‐phase V79 Chinese hamster cells by Method 4 (24 hr at 25°C + 1.5 hr at 37°C + 24 hr at 25°C + trypsin + 3 hr at 37°C) or by Method 3 (48 hr at 25°C + trypsin + 3 hr at 37°C) make mammalian V79 cells significantly m