Induction of tetraploid DNA content by mutant simian virus 40 T antigen that has reduced complex formation with p53
✍ Scribed by John M. Lehman; Mary Beth Albano Perry; Judith Laffin
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 486 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
✦ Synopsis
The 402 mutants (DE, DH, DN) of simian virus (SV) 4 0 form reduced levels of p53-T antigen complexes or no complexes in lytically infected cells (CV-1 cells) relative to wild-type (wt) virus when assayed by immunoprecipitation. When CV-1 cells were infected with the 402 mutants, the cells were induced into multiple rounds of DNA synthesis without mitosis, resulting in a large population of cells with >G2 (tetraploid) DNA content similar to wt virus. The levels of T antigen and p53 per cell that were determined by flow cytometry were similar to wt lytically infected cells, with the levels of T antigen increasing as the infection proceeded. The p53 increased as the levels of T antigen increased, similar to a wt infection. These studies demonstrate that, in lytically infected cells with reduced p53-T antigen complex formation, the cells are induced into multiple rounds of DNA synthesis.