𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction of systemic immunity by expression of interleukin-23 in murine colon carcinoma cells

✍ Scribed by Yan-Qing Wang; Shin-ichi Ugai; Osamu Shimozato; Ling Yu; Kiyoko Kawamura; Hiroshi Yamamoto; Taketo Yamaguchi; Hiromitsu Saisho; Masatoshi Tagawa

Publisher
John Wiley and Sons
Year
2003
Tongue
French
Weight
132 KB
Volume
105
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Interleukin‐23 (IL‐23), a novel cytokine composed of a newly identified p19 molecule and the p40 subunit of IL‐12, can stimulate the proliferation in vitro of memory T cells. We examined whether Colon 26 murine colon carcinoma cells that were retrovirally transduced with the p19‐linked p40 gene (Colon 26/IL‐23) could produce antitumor effects in inoculated mice. The growth of Colon 26/IL‐23 tumors developed in immunocompetent mice was significantly retarded and the tumors disappeared thereafter. Spleen cells from the mice that received Colon 26/IL‐23 cells produced significant amounts of interferon‐γ, when they were cultured with irradiated Colon 26 but not irrelevant cells. Depletion of CD8^+^ T cells suppressed the production of interferon‐γ. The mice that had rejected Colon 26/IL‐23 tumors were resistant to subsequent challenge of parent but not irrelevant tumor cells. Colon 26/IL‐23 tumors were not rejected in nude mice but the growth was retarded compared to parent tumors. Treatment of nude mice with anti‐asialo GM~1~ antibody did not influence the growth of Colon 26/IL‐23 tumors. These data suggest that expression of IL‐23 in tumors produces T cell‐dependent antitumor effects and induces systemic immunity. © 2003 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Modulation of interleukin-18 expression
Modulation of interleukin-18 expression in human colon carcinoma: Consequences for tumor immune surveillance
✍ Franck Pagès; Anne Berger; Berthold Henglein; Bernard Piqueras; Claire Danel; Fr 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 French ⚖ 291 KB 👁 2 views

The production in colon cancer of interferon-gamma (IFN-␥), a type-1 T-helper (TH1) cytokine, is considered as a marker of good prognosis. We asked whether interleukin-18 (IL-18), which strongly induces IFN-␥ and regulates Fas ligand (Fas-L)-dependent cytotoxicity, may play a role in colon homeostas

Induction of GADD gene expression by phe
Induction of GADD gene expression by phenethylisothiocyanate in human colon adenocarcinoma cells
✍ Anna Powolny; Keiko Takahashi; Robin G. Hopkins; George Loo 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB

Phenethylisothiocyanate (PEITC), a potential cancer chemopreventive agent, induces colon cancer cell death, but the mechanism is not entirely clear. Therefore, the aim of this study was to further clarify the molecular effects of PEITC in causing death of human colon adenocarcinoma cells. When incub

Expression of IL-27 in murine carcinoma
Expression of IL-27 in murine carcinoma cells produces antitumor effects and induces protective immunity in inoculated host animals
✍ Masako Chiyo; Osamu Shimozato; Ling Yu; Kiyoko Kawamura; Toshihiko Iizasa; Takeh 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 176 KB

## Abstract A novel cytokine interleukin‐27 (IL‐27), composed of p28 and Epstein‐Barr virus‐induced gene 3 (EBI3), is produced from activated dendritic cells and is involved in an early phase of T‐helper type I differentiation. We examined whether Colon 26 murine colon carcinoma cells that were ret

Kinetics of costimulatory molecule expre
Kinetics of costimulatory molecule expression by T cells and dendritic cells during the induction of tolerance versus immunity in vivo
✍ Kristin Hochweller; Stephen M. Anderton 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 443 KB 👁 1 views

## Abstract Steady‐state dendritic cells (DC) present peptide‐MHC complexes to T cells in a tolerogenic manner, presumably because of deficient costimulation. However, it is clear that the path to tolerance involves initial T cell activation, suggesting that the deficit may lie in late‐acting costi

Involvement of the immune response in th
Involvement of the immune response in the cure of metastatic murine CT-26 colon carcinoma by low electric field-enhanced chemotherapy
✍ Alexander Plotnikov; Thomas Tichler; Rafi Korenstein; Yona Keisari 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 French ⚖ 232 KB

## Abstract Low electric field cancer treatment‐enhanced chemotherapy (LEFCT‐EC) is a new treatment modality that combines chemotherapeutic agents and low electric field stimulation. LEFCT‐EC was found to destroy malignant mouse tumors and cause massive death of tumor cells. This may enable the imm

Induction of apoptosis in human colon ca
Induction of apoptosis in human colon carcinoma cells HT29 by sublethal cryo-injury: Mediation by cytochrome c release
✍ Akira Hanai; Weng-Lang Yang; T.S. Ravikumar 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 487 KB

Cryosurgery is an emerging treatment for human solid tumors, notably colorectal liver metastasis. Cryosurgical procedures generate a thermal gradient of from at least -50 degrees C at the center of the tumor being treated to about 0 degrees C at the periphery. Cell death occurs by necrosis in the ce