Induction of somatic embryogenesis and embryo development inRumex acetosellaL.

✍ Scribed by Ljubinka Ćulafić; SneŽana Budimir; Radmila Vujičić; Mirjana Nešković

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 968 KB
• Volume: 11
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00041846

✦ Synopsis

Axillary buds of the dioecious plant Rumex acetosella L. were isolated and cultured in vitro. The callus tissue which developed at the basal parts of the explants displayed a high capacity for shoot formation. This morphogenetic pattern was predominant on Murashige and Skoog (MS) medium supplemented with 2% sucrose, 2.2 mg 1-~ benzylaminopurine and 0.17 mg 1 -a indole-3-acetic acid. Somatic embryogenesis was induced when the osmolality of the medium was increased by adding 6% sucrose instead of 2%, or hexitols in addition to 2% sucrose. Most of the embryogenic calli were formed on the basal parts of leaf laminae and bracts. Development and maturation was strongly promoted by transferring the tissue to a solid or liquid medium lacking benzylaminopurine and indole-3-acetic acid and supplemented with 10mgl -~ gibberellic acid. The embryos germinated and developed into normal rosette plants when transferred to vermiculite moistened with hormone-free, half-strength MS salt solution. The histology of successive embryogenic stages is presented.