The fluorescence-plus-Giemsa (FPG) technique for analysis of sister chromatid exchange (SCE) is widely used as an assay for mutagenic carcinogens. There is very little information, however, on whether incorporation of the bromodeoxyuridine (BrdU) necessary for visualization of SCEs affects the sensi
Induction of sister chromatid exchanges by 5-fluorodeoxycytidine: correlation with DNA methylation
✍ Scribed by Jasemine Choy Chambers; J. Herbert Taylor
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 442 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0009-5915
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## Abstract The frequency of sister chromatid exchange (SCE) in WI‐38 cells was estimated by the 5‐bromodeoxyuridine (BrdUrd)‐dye technique after one hour's exposure to cyclophosphamide (CY), N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG), 4‐nitroquinoline‐1‐oxide (4NQO), and maleic hydrazide (MH) wit
The inducibility of sister chromatid exchanges (SCEs) by benzo(a)pyrene (6P) was studied in cultured peripheral blood lymphocytes of I5 untreated lung cancer patients and 25 healthy persons including I I high-and 14 low-cancer-risk individuals tentatively classified by the familial history of lung c