INDUCTION OF SIALYL LEWISX ON THE SURFACE OF CULTURED RAT VASCULAR ENDOTHELIAL CELLS AND CARDIAC MYOCYTES BY HYPOXIA/REOXYGENATIONIN VITRO
✍ Scribed by SEKO, YOSHINORI; ENOKAWA, YOSHIFUMI; TAMATANI, TAKUYA; KANNAGI, REIJI; YAGITA, HIDEO; OKUMURA, KO; YAZAKI, YOSHIO
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 517 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0022-3417
No coin nor oath required. For personal study only.
✦ Synopsis
Neutrophils adhere to and roll on vascular endothelial cells (VECs) through interaction of selectins and their carbohydrate ligands in the early stages of inflammation; this adhesion is then later strengthened through interaction of integrins on neutrophils with intercellular adhesion molecule-1 (ICAM-1) on endothelial cells. Recent, as yet unpublished studies showed that myocardial ischaemialreperfusion caused rapid expression of sialyl LewisX (SLeX), one of the carbohydrate ligands of selectins, on VECs and cardiac myocytes and that an anti-SLeX monoclonal antibody (MAb) significantly reduced myocardial reperfusion injury in v i m In the present study, to investigate whether or not ischaemialreperfusion itself can induce the expression of SLeX on VECs and cardiac myocytes, the expression of SLeX on cultured rat VECs and cardiac myocytes was examined by treatment with hypoxialreoxygenation in vitvo, because ischaemial reperfusion stimuli may partly be due to hypoxialreoxygenation. The expression of SLeX was induced rapidly and temporarily on the surface of cultured rat cardiac myocytes and VECs by hypoxialreoxygenation in vitvo. This strongly suggests that the expression of SLeX on the surface of myocardial cells is induced initially and directly by ischaemialreperfusion, which results in the rolling attachment of neutrophils in the early stages of myocardial reperfusion injury.