Induction of reproductive system tumors in mice by N6-(methylnitroso)-adenosine and a tumorigenic effect of its combined precursors
✍ Scribed by Lucy M. Anderson; Alfredo Giner-Sorolla; Jeffrey H. Greenbaum; Kathleen Last-Barney; John M. Budinger
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- French
- Weight
- 274 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Interaction of the naturally‐occurring nucleoside, N^6^‐methyl adenosine, with nitrite, a reaction that occurs readily under acidic conditions, results in the formation of a nitrosamine, N^6^‐(methylnitroso) adenosine [m^6^(NO)Ado]. This nitrosamine was given in the drinking water (1 mM solution) of non‐inbred Swiss mice from 3 weeks of age until death. It caused a significant increase in the incidence of primary lung tumors, compared with controls. It also induced reproductive tract tumors in 80% of the exposed females, including mammary tumors in 60% and uterine tumors in 25%. The precursors of m^6^(NO) Ado, m^6^Ado and nitrite, did not elevate tumor incidence when given singly, but when administered together resulted in a significant increase in numbers of lung tumors in the males. The nitrosamine base, N^6^‐(methylnitroso)adenine, was found to be a less potent carcinogen than m^6^(NO)Ado, causing lung tumors only in males and possibly a few mammary tumors in females. These results indicate the in vivo formation of a carcinogen from m^6^Ado and nitrite, and show that m^6^(NO)Ado induces neoplasms in the reproductive system of mice, an unusual target for a N‐nitroso carcinogen.