Induction of Multinucleated Giant Cell Formation from In Vitro Culture of Human Monocytes with Interleukin-3 and Interferon-γ: Comparison with Other Stimulating Factors

Recombinant murine granulocyte-macrophag

Recombinant murine granulocyte-macrophage (GM) colony-stimulating factor supports formation of GM and multipotential blast cell colonies in culture: Comparison with the effects of interleukin-3

✍ Kenichi Koike; Makio Ogawa; James N. Ihle; Takaji Miyake; Tomoe Shimizu; Atsushi 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 808 KB

We studied the effects of murine recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) on murine hemopoiesis in methylcellulose culture. The GM-CSF was purified from cultures of Saccharomyces cerevisiae transfected with a cloned murine GM-CSF cDNA. In cultures of spleen cells from no