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Induction of metallothionein synthesis in cultured cells derived from rabbit kidney

✍ Scribed by Shinji Koizumi; Toshio Sone; Masami Kimura

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
641 KB
Volume
125
Category
Article
ISSN
0021-9541

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✦ Synopsis

Metallothionein (MT) s nthesis in rabbit kidney-derived RK-13 cells was studied. In response to Cd'+, RK-13 cells synthesized proteins closely similar in chromatographic and electrophoretic behaviors to the liver MTs induced in Cd2+-injected rabbit. These proteins were specifically immunoprecipitated by anti-mouse liver MT-II serum. The rate of RK-13 thionein (apoprotein of MT) synthesis rapidly increased after exposure to 1 p g h l of Cd2+, and reached the maximum in 7 h. The dose-response curve for the synthesis was biphasic; a sharp increase u p to 0.5 pgirnl and a slower increase at higher concentrations. RK-13 cells retained kidney-specific properties in terms of responsiveness of thionein synthesis to inducers; The MTs were inducible also by Zn2+ and probably by Hg2+, but not by dexamethasone. This system would therefore be a useful model in vitro for studying the regulation of MT synthesis in kidney cells.

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