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Induction of Id2 expression by cardiac transcription factors GATA4 and Nkx2.5

✍ Scribed by Joong-Yeon Lim; Won Ho Kim; Joon Kim; Sang Ick Park

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
386 KB
Volume
103
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

Inhibitor of differentiation/DNA binding (Id) proteins function as a regulator of helix‐loop‐helix proteins participating in cell lineage commitment and differentiation. Here, we observed a marked induction of Id2 during cardiomyocyte differentiation from P19CL6 murine embryonic teratocarcinoma stem cells, prompting us to investigate the upstream regulatory mechanism of Id2 induction. Computer analysis of Id2 promoter and subsequent electrophoretic mobility shift assay revealed several binding sites for GATA4 and Nkx2.5 within the Id2 promoter. By further deletion and mutation analysis of the respective binding site, we identified that two motifs located at βˆ’497/βˆ’502 and βˆ’264/βˆ’270 were functionally important for Id2 promoter activation by GATA4 and Nkx2.5, respectively. Overexpression of GATA4 and/or Nkx2.5 induced not only Id2 promoter activity but also Id2 protein expression. Additionally, Id proteins significantly inhibit the GATA4 and Nkx2.5‐dependent transcription, suggesting Id proteins may play a regulatory role in cardiogenesis. Collectively, our results demonstrate that GATA4 and Nkx2.5 could be one of the upstream regulators of Id2. J. Cell. Biochem. 103: 182–194, 2008. Β© 2007 Wiley‐Liss, Inc.

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