Induction of human leukocyte antigen (HLA)-A2-restricted and MAGE-3-gene-derived peptide-specific cytolytic T lymphocytes using cultured dendritic cells from an HLA-A2 esophageal cancer patient
✍ Scribed by Kanaoka, Shunji; Yamasaki, Seiji; Okino, Takashi; Inoue, Naoya; Shimada, Yutaka; Kaneko, Midori; Otaka, Akira; Fujii, Nobutaka; Imamura, Masayuki
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 154 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0022-4790
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✦ Synopsis
Background and objectives:
Using peripheral blood mononuclear cells (pbmcs) from a 10-year survivor with established human leukocyte antigen (hla)-a2(+) and mage-3(+) esophageal cancer cell line (kyse-170), we examined the induction of hla-a2-restricted and mage-3-gene-derived peptide (flwgpralv, amino acids 271-279)-specific cytolytic t lymphocytes (ctls).
Methods:
Autologous dendritic cells (dcs) cultured with granulocyte-macrophage colony stimulating factor and interleukin-4 were used as antigen presenting cells. pbmcs were stimulated by peptide-pulsed dcs in vitro.
Results:
Pbmc cocultured with flwgpralv-pulsed dcs could induce the relevant peptide-specific ctls, which had tumor necrosis factor production and specific cytotoxicity against relevant peptide-pulsed autologous dcs (34%, effector:target ratio = 40:1). moreover, they showed specific cytotoxicity against the autologous esophageal cancer cell line kyse-170 (17%, effector:target ratio = 40:1).
Conclusions:
These results suggest that flwgpralv-pulsed cultured dcs would be a potent candidate for peptide vaccine against hla-a2(+) and mage-3(+) esophageal cancer.