Induction of human interleukin 1 by bacterial and synthetic lipid A

✍ Scribed by Harald Loppnow; Lore Brade; Helmut Brade; Ernst Theodor Rietschel; Shoichi Kusumoto; Tetsuo Shiba; Hans-Dieter Flad

• Publisher: John Wiley and Sons
• Year: 1986
• Tongue: English
• Weight: 452 KB
• Volume: 16
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830161013

✦ Synopsis

Induction of human interleukin 1 by bacterial and synthetic lipid A*

Lipopolysaccharides (LPS) are well known inducers of interleukin 1 (IL 1). Here we show that synthetic heptaacyl Salmonella minnesota (compound 516) and synthetic E. coli type (compound 506) lipid A , as well as monodephospho part structures thereof, are able to induce IL 1 production in human mononuclear cells. The 4'-monodephospho part structure (compound 505) was found to be the most active IL 1 inducer as compared to compound 506 and the 1-monodephospho part structure (compound 504). Synthetic lipid A precursor Ia, lacking nonhydroxylated fatty acids (compound 406), and its 1or 4'-monodephospho part structures (compounds 404 and 405) did not induce IL 1 production. IL2 is not produced during stimulation with the synthetic compounds. IL 1 activity was partially heat labile and could be inhibited by a rabbit antiserum against human leukocytic pyrogen. These results show that the lipid A component of LPS is active in inducing IL 1 and that the presence of 3-acyloxyacyl residues is necessary for IL 1 induction.