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Induction of heterothallic strains and their genetic and physiological characterization in a homothallic strain of the yeastSaccharomyces exiguus

โœ Scribed by Taisuke Hisatomi; Naohiko Yanagishima; Isao Ban-no

Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
468 KB
Volume
10
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

We isolated heterothallic strains from a homothallic strain of S. exiguus by mutagenization with UV or ethylmethanesulfonate (EMS). A gene, not linked to the mating-type locus, was found to control homothallism in the yeast, as in S. cerevisiae. alpha Pheromone of S. exiguus (alpha se pheromone) induced formation of large pear-shaped cells (shmooing) in alpha strains of S. exiguus, S. cerevisiae, and S. kluyveri, and sexual agglutinability of an inducible alpha strain of S. cerevisiae. alpha se Pheromone is a peptidyl substance a little different from alpha pheromone of S. cerevisiae. alpha Pheromone of S. exiguus acts only on alpha cells of S. exiguus. Contrary to the above results, neither sexual agglutination nor zygote formation occurred among these three Saccharomyces yeasts.

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