Induction of germline-length mutations at the minisatellites PC-1 and PC-2 in male mice exposed to polychlorinated biphenyls and diesel exhaust emissions
✍ Scribed by Mona Hedenskog; Michael Sjögren; Håkan Cederberg; Ulf Rannug
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 96 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
PC-1 and PC-2 are hypervariable mouse minisatel-posed to diesel exhausts and/or polychlorinted bilites. The rates of spontaneous germline-length muta-phenyls (PCB) were investigated for effects on the tion have been shown to vary between different germline mutation frequenallele lengths in parents mouse strains. PC-1 is composed of GGCAG repeat and offspring. For PC-1 significantly higher mutaunits and PC-2 of GGCAGGA. Minisatellites fre-tion frequencies were found in males treated with quently mutate by gaining or losing repeat units. diesel exhausts / PCB (6 of 35 alleles) and with Such length mutations in mini-and microsatellites PCB alone (6 of 51 alleles) as compared to the have been associated with human disease and may males in the control group (0 of 43 alleles). The therefore be an important endpoint in genetic toxic-mutation frequency in the diesel exhaust group was ity testing. Carcinogenic activity of many chemicals not significantly increased (2 of 43 alleles). For PCis associated with their ability to induce heritable 2 the only mutation found occurred in the PCB group mutations. Since minisatellites are highly prone to (1 of 51 alleles). This in vivo study demonstratesmutate to new lengths, which can be assayed by for the first time-chemically induced minisatellite Southern analysis, we used this method to detect mutations in the germline.