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Induction of G- and R-banding in human chromosomes by the demethylating agentS-adenosyl-l-homocysteine

✍ Scribed by S. F. Cabo; C. Sentís; J. Fernández-Piqueras

Publisher
Springer
Year
1992
Tongue
English
Weight
238 KB
Volume
97
Category
Article
ISSN
1432-119X

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✦ Synopsis

In this report we describe the procedure of growing human lymphocytes with the demethylating agent S-adenosyl-L-homocysteine (SAH). After this treatment, which is not toxic for cell survival, both R- and G-banding were obtained by new experimental procedures: R-bands have been directly demonstrated with the GC-specific fluorochrome chromomycin A3 without the necessity of any AT-specific counterstaining agent; simultaneous G-banding and active nucleolar organizer regions have been obtained by silver impregnation of chromosomes and subsequent Giemsa staining. These results suggest a possible relationship between local differences in DNA methylation and the determination of the banded chromosome structure.

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