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Induction of embryonic stem cells to hematopoietic cellsin vitro

โœ Scribed by Ling Xu; Chunping Qiao; Shaoliang Huang; Shunong Li; Xuan Wu

Publisher
Springer
Year
2000
Tongue
English
Weight
440 KB
Volume
45
Category
Article
ISSN
1001-6538

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A retroviral gene trap vector (U3Tkneo) that selects for integrations in or near expressed 5ะˆ exons has been used to identify genes that are repressed during hematopoietic differentiation of mouse totipotent embryonic stem cells. The vector contains coding sequences for an HSV-thymidine kinase/neomy