Induction of chromosomal aberrations in mouse zygotes by acrylamide treatment of male germ cells and their correlation with dominant lethality and heritable translocations
✍ Scribed by Francesco Marchetti; Xiu Lowe; Jack Bishop; Andrew J. Wyrobek
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 78 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
The objectives of this research were: 1) to investi-PAINT/DAPI analysis revealed that: 1) AA-induced gate the time course of the cytogenetic defects in-chromosomal breaks occurred at random, and 2) duced by acrylamide (AA) treatment (5 1 50 mg/ the frequencies of symmetrical and asymmetrical kg) of male germ cells in first-cleavage zygote meta-exchanges were similar at all mating days, except phases using PAINT/DAPI analysis, and 2) to char-9.5 d after AA treatment, where significantly (P õ acterize the correlation between chromosomal ab-0.02) more asymmetrical aberrations were found. errations at first cleavage, dominant lethality, and Furthermore, the proportions of zygotes carrying unheritable translocations. PAINT/DAPI analysis em-stable and stable chromosomal aberrations folploys multicolor fluorescence in situ hybridization lowed a similar post-treatment time course as the painting plus DAPI staining to detect both stable and proportions of dominant lethality among embryos unstable chromosomal aberrations at first-cleavage and heritable translocations among offspring. metaphase of the zygote. High levels of chromosom-These findings indicate that PAINT/DAPI analysis ally defective zygotes were detected after mating of zygotic metaphases is a promising method for at all postmeiotic stages (20-190-fold, P õ 0.001). detecting male germ cell mutagens capable of in-Early spermatozoa (6.5 d post-treatment) were the ducing chromosomal aberrations and for evaluatmost sensitive, with 76% of the zygotes carrying ing the associated risks for embryonic loss and balcytogenetic defects. A significant 10-fold increase anced translocations at birth. Environ. Mol. Mutawas also detected 27.5 d post-treatment, indicating gen. 30:410-417, 1997 ᭧ 1997 Wiley-Liss, Inc. that AA had a cytogenetic effect on meiotic stages.