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Induction of callus from axillary buds of taro (Colocasia esculentavar.esculenta, Araceae) and subsequent plantlet regeneration

โœ Scribed by Tim W. Yam; John L. P. Young; Kap P. L. Fan; J. Arditti

Publisher
Springer
Year
1990
Tongue
English
Weight
598 KB
Volume
9
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

Axillary buds of taro (Colocasia esculenta var. esculenta, Araceae) cultured on half strength Murashige-Skoog medium (HMS) containing taro extract (HMSTE) and 2, 4, 5-trichlorophenoxyacetic acid produce a compact, hard, slow growing callus which is not very active morphogenetically and produces only a few plantlets. When cultured on HMSTE plus 5 mg 1(-1) each of naphthaleneacetic acid and benzyl adenine (HMSNB) the buds produce a fast growing, friable and morphogenetically active callus. Meristematic regions form on the friable callus after 30 days on HMSNB. If transferred to HMSTE at this point the callus gives rise to plantlets. Addition of taro extract to the media is required for the culture of buds, induction of callus and plantlet regeneration.

๐Ÿ“œ SIMILAR VOLUMES

Callus formation and plantlet developmen
Callus formation and plantlet development from axillary buds of taro
โœ Tim Wing Yam; Emma L. Webb; Joseph Arditti ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 604 KB

Excised lateral buds o f taro [Colocasia esculenta var. esculenta (L.) A . F . Hill] developed into plantlets and f o r m e d callus if cultured on m e d i a containing taro extract. ~-Naphthaleneacetic acid enhanced the process but only if taro extract was also present. The tissue requirements for