Induction of c-fos and c-jun protooncogenes expression by formaldehyde-releasing and epoxy resin-based root-canal sealers in human osteoblastic cells

✍ Scribed by Huang, Fu-Mei ;Hsieh, Yih-Shou ;Tai, Kuo-Wei ;Chou, Ming-Yung ;Chang, Yu-Chao

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 353 KB
• Volume: 59
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.10022

✦ Synopsis

Abstract

An important requirement for a root‐canal sealer is biologic compatibility; most evaluations have focused on general toxicological and local tissue irritating properties. There is only scant information about mutagenicity or carcinogenicity testing for root‐canal sealer. It has been shown that c‐fos and c‐jun are induced rapidly by a variety of chemical and physical stimuli. Numerous works have extensively investigated the induction mechanisms of c‐fos and c‐jun protooncogenes by these agents; however, little is known about the induction of cellular signaling events and specific gene expression after cell exposure to root‐canal sealers. Therefore, we used osteoblastic cell line U2‐OS to examine the effect of zinc‐oxide eugenol‐based (N2 and Endomethasome), epoxy resin‐based (AH Plus), and calcium hydroxide‐based (Sealapex) root‐canal sealers on the expression of c‐fos and c‐jun protooncogenes to understand in more detail the molecular mechanisms of root‐canal sealer‐induced genotoxicity. The cytotoxicity decreased in an order of N2 > Endomethasome > AH Plus > Sealapex. In addition, N2, Endomethasome, and AH Plus rapidly induced c‐jun and c‐fos mRNA levels in cells. However, Sealapex did not induce c‐jun and c‐fos mRNA expression at detectable levels all time points. Taken together, persistent induction of c‐jun and c‐fos protooncogenes by formaldehyde‐releasing and epoxy resin‐based root‐canal sealers may be distributed systemically via apex to cause some unexpected adverse effects on human beings. These data should be taken into consideration when choosing a root‐canal sealer. © 2001 Wiley Periodicals, Inc. J Biomed Mater Res 59: 460–465, 2002