𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction of apoptosis by ribosome-inactivating proteins and related immunotoxins

✍ Scribed by Andrea Bolognesi; Pier Luigi Tazzari; Fabiola Olivieri; Letizia Polito; Brunangelo Falini; Fiorenzo Stirpe

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
702 KB
Volume
68
Category
Article
ISSN
0020-7136

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✦ Synopsis

lmmunotoxins have been prepared with 3 riborome-inactivating proteins (RIPs), namely, momordin, .pokeweed anti-viral protein from seeds (PAP-S) and saporin, linked to the Ber-H2 monoclonal antibody directed against the CD30 antigen of human lymphocytes. Either the RIPS or the immunotoxins induced apoptosis in the CD30+ W O cell line, as shown by the morphological aspects of the cells, by the DNA fragmentation visible at the electrophoresis, and by the formation of DNA breaks evidenced by 2 cytofluorometric techniques (propidiumiodide staining and fluoresceine-isothiocyanate conjugate dUTP incorporation). The ACSo (concentration causing apoptosis in 50% of the cells) is in the range to lo-' M in the case of RIPS, and lo-" to 1O-Io M in the case of the immunotoxins.

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