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Induction of alcohol dehydrogenase null mutants in the mediterranean fruit flyCeratitis capitata

✍ Scribed by M. E. Riva; A. S. Robinson


Publisher
Springer
Year
1986
Tongue
English
Weight
501 KB
Volume
24
Category
Article
ISSN
0006-2928

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✦ Synopsis


Alcohol dehydrogenase null mutants have been induced with X rays in Ceratitis capitata, for use in a genetic sexing system. A combination of selective medium and electrophoretic screening was used to identify these mutants. The results indicate that they are probably large deletions since no homozygotes were found for any of the induced mutants.


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The toxicity of the first eight primary alcohols and of four secondary alcohols was compared in a wild-type strain (having active ADH) and an ADH-negative mutant. Differences between LC50 measured in the two strains allowed an evaluation of the biological activity of the enzyme. In vitro, ADH is mai