𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction kinetics of mutagenic DNA repair activity in E. coli following ultraviolet irradiation

✍ Scribed by Defais, Martine ;Caillet-Fauquet, Perrine ;Fox, Maurice S. ;Radman, Miroslav

Publisher
Springer
Year
1976
Tongue
English
Weight
593 KB
Volume
148
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Ultraviolet mutagenesis of phage gamma is produced by host functions which are inducible by ultraviolet irradiation of the host cell. Induction kinetics and the half life of the inducible mutagenic DNA repair (SOS-repair) in E.coli have been determined using phage gamma assays. At 37 degrees C, both mutagenic and repair activities are maximal approximately 30 min following irradiation and decay with a half life of approximately 30 min. The presence of 100 mug/ml chloramphenicol during the first 40 min after irradiation completely abolishes induction of repair and mutagenesis. The ultraviolet induction pattern of SOS repair very much resembles that of gamma prophage in lysogenic induction (Monk and Kinross, 1975).

πŸ“œ SIMILAR VOLUMES

Repair and recombination of nonreplicati
Repair and recombination of nonreplicating UV-irradiated phage DNA in E. coli III
✍ Hays, John B. ;Lee, Euihum πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 851 KB

The question of whether induction of the SOS response in Escherichia coli increases the efficiency of excision repair was addressed by measuring repair of UV-damaged nonreplicating lambda phage DNA in previously irradiated bacteria. Prior UV irradiation of lex+ bacteria enhanced both the rate of reg

Accelerated repair and reduced mutagenic
Accelerated repair and reduced mutagenicity of oxidative DNA damage in human bladder cells expressing the E. coli FPG protein
✍ Monica Ropolo; Alessandro Geroldi; Paolo Degan; Virginia Andreotti; Simona Zupo; πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 French βš– 703 KB

## Abstract Repair of some oxidized purines such as 8‐oxo‐7,8‐dihydroguanine (8‐oxoG) is inefficient in human cells in comparison to repair of other major endogenous lesions (__e.g.__ uracil, abasic sites or oxidized pyrimidines). This is due to the poor catalytic properties of hOGG1, the major DNA