Amino acid transport was studied in primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion technique and maintained as a monolayer in a serum-free culture medium. These cells carried out gluconeogenesis from three carbon precursors (alanine, pyruvate, and lact
Induction and Decay of Glucagon-Induced Amino Acid Transport in Primary Cultures of Adult Rat Liver Cells: Paradoxical Effects of Cycloheximide and Puromycin
โ Scribed by Michael W. Pariza, Fred R. Butcher, Rolf F. Kletzien, Joyce E. Becker and Van R. Potter
- Book ID
- 123654102
- Publisher
- National Academy of Sciences
- Year
- 1976
- Tongue
- English
- Weight
- 906 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/66145
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## Abstract Parenchymal cells from adult rat liver, isolated by a collagenase perfusion technique, have been maintained in primary culture and a detailed study on carbohydrate metabolism carried out over the initial 48โhour culture period. The glucose concentration of the medium exerts a major infl