Inducible reactivation of UV-irradiated cholera phage e5 in Vibrio cholerae MAK757
✍ Scribed by Basu, Rina ;Ghosh, Amit
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 414 KB
- Volume
- 209
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
The survival of UV-irradiated cholera phage e5 was found to increase when the host cells, Vibrio cholerae MAK757, were exposed to a low dose of UV irradiation before phage infection (Weigle reactivation), indicating the existence of a UV-inducible DNA repair pathway (SOS repair) in V. cholerae MAK757. The induction signal generated by UV irradiation was transient in nature and lasted about 20-30 min at 37 degrees C. Maximal Weigle reactivation of the phage was obtained when the host cells were irradiated with a UV dose of 16 J/m2. V. cholerae MAK757 was also found to possess efficient photoreactivation and host cell reactivation of UV-damaged DNA in phage e5.