𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Inducible expression of the gap junction protein connexin43 decreases the neoplastic potential of HT-1080 human fibrosarcoma cells in vitro and in vivo

✍ Scribed by Timothy J. King; Laurie H. Fukushima; Yutaka Yasui; Paul D. Lampe; John S. Bertram

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
264 KB
Volume
35
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Numerous studies have demonstrated a correlation between dysregulation/loss of connexin expression or gap junction intercellular communication (GJIC) function and decreased growth control both in human tumors and tumor cell lines. Likewise, restoration of constitutive connexin expression/function is correlated with increased growth control/decreased tumorigenicity. Here, we show for the first time that inducible restoration of connexin43 (Cx43) expression and GJIC function in a human tumor line of mesenchymal origin (HT‐1080, fibrosarcoma) resulted in a lowered neoplastic potential. Specifically, HT‐1080 cells induced to express Cx43 demonstrated diminished foci formation when in co‐culture with normal fibroblasts, decreased colony formation under anchorage‐independent conditions, and reduced tumor growth when injected into immunodeficient mice. These results, obtained utilizing an inducible system that helps address issues of clonal heterogeneity, strongly implicate Cx43 as a tumor suppressor in human tissue of mesenchymal origin and GJIC as a regulatory mechanism for cellular growth control both in vitro and in vivo. This study also further supports the hypothesis that loss of Cx43/GJIC in human tumors may play an important role in the dysregulation of normal growth control. © 2002 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Doubly mutant mice, deficient in connexi
Doubly mutant mice, deficient in connexin32 and -43, show normal prenatal development of organs where the two gap junction proteins are expressed in the same cells
✍ Houghton, Franchesca D. ;Th�nnissen, Eva ;Kidder, Gerald M. ;Naus, Christian C.G 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 338 KB 👁 2 views

The connexins are a family of proteins that form the intercellular membrane channels of gap junctions. Genes encoding 13 different rodent connexins have been cloned and characterized to date. Connexins vary both in their distribution among adult cell types and in the properties of the channels that

The carboxy-terminal tail of connexin43
The carboxy-terminal tail of connexin43 gap junction protein is sufficient to mediate cytoskeleton changes in human glioma cells
✍ Sophie Crespin; John Bechberger; Marc Mesnil; Christian C. Naus; Wun-Chey Sin 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 466 KB 👁 1 views

## Abstract Connexin43 (Cx43) is a ubiquitously expressed member of the gap junction protein family that mediates gap junction intercellular communication (GJIC) by allowing exchange of cytosolic materials. Previous studies have used Cx43 truncated at the cytoplasmic tail (C‐tail) to demonstrate th

Fibroblast growth factors-5 and -9 disti
Fibroblast growth factors-5 and -9 distinctly regulate expression and function of the gap junction protein connexin43 in cultured astroglial cells from different brain regions
✍ B. Reuss; M. Hertel; S. Werner; K. Unsicker 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 372 KB 👁 2 views

Astroglial cells contribute to neuronal maintenance and function in the normal and diseased brain. Gap junctions formed predominantly by connexin43 (cx43) provide important pathways to coordinate astroglial responses. We have previously shown that fibroblast growth factor (FGF)-2, which occurs ubiqu