✍ Scribed by Edward J. Eyring; Howard Kraus; Jen Tsi Yang
No coin nor oath required. For personal study only.
Chondroitin sulfate C (CSC) and acrdiine orange (AO) formed two types of complexes a t neutral pH, depending upon the order of mixing. The induced optical activity of AO was much more pronounced when the polysaccharide wm added to dye than the dye to polymer (final concentration of dye was 5 X 105M). The difference in aggregation of the dye molecules is believed to be responsible for the observed peculiarities. The Cotton effects of the CSC-todye solution displayed a sharp inversion near 59°C. and the profile a t 76°C. was almost a mirror image of that at room temperature. At pH 1.3, however, the order of mixing became unimportant, suggesting that, the carboxylate on the polysaccharide was involved more intimately than were sulfates in the peculiarities of the Cotton effects.
📜 SIMILAR VOLUMES
Chondroitin sulfate C (CSC), isolated from shark vertebral mucoprotein, has a molecular weight of 5.2 x lOe5.5 X lo4 in both NaCl and CaC12 solutions. Optical rotatory dispersion and circular dichroism of CSC reveal a strong, negative, optically active band near 210 mp, arising from the carboxylate