Induced diploid gynogenesis and polyploidy in the ornamental (koi) carpCyprinus carpioL.

✍ Scribed by N. B. Cherfas; Y. Peretz; N. Ben-Dom; B. Gomelsky; G. Hulata

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 478 KB
• Volume: 89-89
• Category: Article
• ISSN: 0040-5752
• DOI: 10.1007/bf00225155

✦ Synopsis

The results of a series of experiments conducted in our laboratory on the ornamental common carp (koi), aimed at optimizing heat-shock chromosome-set manipulation procedures, are described. The timing of heat-shock initiation was expressed in the relative unit of embryological age (τ0) in order to standardize this parameter, the absolute time for heat-shock initiation being calculated from duration of one τ0 at two different pre-treatment water temperatures. Heat shocks were applied within the periods of 0.05-0.60 τ0 and 1.20-2.20 τ0 which, respectively, cover the successive phases of the 2nd meiotic division and the 1st cleavage. The highest production of diploid gynogenetic offspring was observed when heat shocks were initiated at 0.15-0.25 τ0 and at 1.5 τ0, after insemination, corresponding to anaphase of meiosis-II, and metaphase of the 1st cleavage, respectively. Similar results were obtained irrespective of the different pre-treatment water temperatures, thus confirming the possibility of standardizing heat-shock timing by τ0.