Induced circular dichroism of acridine orange bound to double-stranded RNA and transfer RNA

✍ Scribed by Mitsuo Zama; Sachiko Ichimura

• Publisher: Wiley (John Wiley & Sons)
• Year: 1976
• Tongue: English
• Weight: 364 KB
• Volume: 15
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1976.360150907

✦ Synopsis

Abstract

The induced circular dichroism (CD) in the visible region of acridine orange bound to the double‐stranded RNA from cytoplasmic polyhedrosis virus and to yeast tRNA has been measured as a function of RNA phosphate‐to‐dye ratio (P/D), under the conditions of 0.01 M Na^+^ at pH 7.0. The shape of the CD spectrum of acridine orange bound to the double‐stranded RNA was quite different from the spectrum of the dye bound to DNA. The CD spectral features of acridine orange bound to the double‐stranded regions in tRNA closely resembled those of the double‐stranded RNA‐dye complex, suggesting that the dyes bind similarly to the two RNA's. It was further found that the CD spectrum of the tRNA‐dye complex at sufficiently high P/D ratios, which is assignable to monomeric, intercalated dye to the base‐paired parts in tRNA, is also distinct from the corresponding spectrum of the DNA‐dye complex.