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Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

✍ Scribed by Tsutomu Arakawa; Kouhei Tsumoto; Daisuke Ejima; Yoshiko Kita; Yasushi Yonezawa; Masao Tokunaga

Book ID: 113681877
Publisher: Elsevier Science
Year: 2007
Tongue: English
Weight: 225 KB
Volume: 70
Category: Article
ISSN: 0165-022X
DOI: 10.1016/j.jbbm.2006.12.001

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## Abstract A library of cold shock protein B (CspB) mutant variants was employed to study protein binding affinity and preferred orientations in cation exchange chromatography. Single site mutations introduced at charged amino acids on the protein surface resulted in a homologous protein set with