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Indole derivatization procedures for electron capture negative chemical ionization mass spectrometry: Identification of 1-methyl-1,2,3,4-tetrahydro-β-carboline in rat brain and lung

✍ Scribed by Talmage R. Bosin; Kym F. Faull


Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
528 KB
Volume
18
Category
Article
ISSN
1076-5174

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✦ Synopsis


Procedures have been developed for the isolation of pharmacologically active indole compounds from biological samples and for the introduction of electron-capturing groups, peotafluorohenzyl and trifluoroacetyl, onto the indole nitrogen atom. The resulting derivatives have good gas chromatographic properties and strong elestron affinities which make them highly suitable for detection by electron capture negative chemical ionization mass spectrometry. These procedures were used to identify l-metbyl-l,2,3,4tetrahydro-~arboline as a component of rat brain and lung.