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Indirect fluorimetric detection and quantification in capillary zone electrophoresis of inorganic anions and necleotides

✍ Scribed by Larry Gross; Edward S. Yeung

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
622 KB
Volume
480
Category
Article
ISSN
1873-3778

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✦ Synopsis

The combination of indirect fluorescence detection and separation by capillary zone electrophoresis is discussed. Differences in instrument configuration, compared with earlier experiments, resulted in a reversed peak elution order. Separation of a S-triphosphate nucleotide from its corresponding diphosphate and monophosphate homologues can be accomplished in 5 min by this method. A quantitative comparison is made between the injection methods of electromigration and injection by gravity flow for samples of inorganic anions diluted in water with varying total concentrations. With injection by electromigration, the amount of sample injected varies significantly with the total ionic concentration of the sample.

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Quantitative capillary zone electrophore
Quantitative capillary zone electrophoresis of inorganic anions
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## Abstract Despite the availability of commercial capillary electrophoresis systems for over tenΒ years, where quantitative analysis is required, capillary zone electrophoresis (CZE) has often failed to replace ion chromatography as the method of choice for a large number of analytes, not least ino