Indirect and lactation-associated changes in renal alkaline phosphatase of newborn rats prenatally exposed to cadmium chloride
✍ Scribed by A. M. Saillenfait; J. P. Payan; M. Ban; J. de Ceaurriz
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 594 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0260-437X
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✦ Synopsis
Pregnant Sprague-Dawley rats were intraperitoneally injected with physiological saline solution (vehicle) or cadmium chloride (CdCI2) at 2.5 mg kg-' body wt. on days 8, 10, 12 and 14 of gestation. Offspring were examined for renal alkaline phosphatase activity (ALP) on postnatal days (PND) 3 and 12, and for kidney metallothionein (MTh) and for liver, kidney and entire gastrointestinal tract IwCd content at birth and on PND 3 and 12. No effects were observed on neonatal survival or on body, liver and kidney weights of pups up to PND 12. Newborns born and fed by mothers exposed to CdC12 during pregnancy exhibited a significant decrease in ALP activity on PND 3. Conversely, no significant changes were observed in newborns lactated by surrogate non-treated mothers. Renal MTh increased with age but was not influenced by maternal treatment. Traces of lwCd were present in the liver at birth (5-7 ng). Thereafter, IwCd was mainly found in the gastrointestinal tract of newborns lactated by their biological mothers (610490 ng on PND 12), with a marginal uptake in the liver (10-12 ng on PND 12). IwCd was not detectable in the kidneys at any age These results show that prenatal exposure to Cd cannot be the sole aetiological agent in the induction of the subtle and transitory changes in renal biochemistry observed in offspring born and fed by female rats intraperitoneally injected with 2.5 mg CdC12 kg-' body wt. on days 8, 10, 12 and 14 of gestation. The results also contradict the role of a direct effect on the kidney.