Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain

✍ Scribed by Juan Pablo Riva Mercadal; Paula Isaac; Faustino Siñeriz; Marcela Alejandra Ferrero

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 127 KB
• Volume: 50
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.200900276

✦ Synopsis

Abstract

A technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min^–1^ mg dry biomass^–1^, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)