Indicator Cell Lines for Detection of Primary Strains of Human and Simian Immunodeficiency Viruses
β Scribed by Marie A. Vodicka; Wei Chun Goh; Lily I. Wu; Mark E. Rogel; Steven R. Bartz; Vicki L. Schweickart; Carol J. Raport; Michael Emerman
- Book ID
- 112271602
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 769 KB
- Volume
- 233
- Category
- Article
- ISSN
- 0042-6822
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No single established cell line was found capable of substituting for primary baboon kidney (PBK) or primary rhesus macacque kidney (PRK) cells for detection of human viruses. Although a panel of cell lines could detect influenza, parainfluenza, and enteroviruses, which are among the most important
## Abstract ## Background Lentiviral vectors allow gene transfer into nonβdividing cells. Further development of these vector systems requires stable packaging cell lines that enable adequate safety testing. ## Methods To generate a packaging cell line for vectors based on simian immunodeficienc