Indicator assay for amino acid decarboxylases
β Scribed by Robert M. Rosenberg; Richard M. Herreid; George J. Piazza; Marion H. O'Leary
- Book ID
- 102988513
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 641 KB
- Volume
- 181
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A general radiochemical method for estimating the activity of amino acid decarboxylases is reported. This method utilizes ion-exchange cartridges to separate unreacted radiolabeled amino acid substrates from product amines, which can then readily be quantitated by liquid scintillation counting. The
## Abstract The development of sensitive and easyβtoβapply highβthroughput screening methods is a common need in modern biocatalysis. With these powerful analytical tools in hands, chemists can easily assess enzyme libraries to identify either novel biocatalysts or improved mutants. Within biocatal