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Increasing the lateral resolution of 4Pi fluorescence microscopes

โœ Scribed by Sandeau, Nicolas; Giovannini, Hugues


Book ID
115390533
Publisher
Optical Society of America
Year
2006
Tongue
English
Weight
584 KB
Volume
23
Category
Article
ISSN
1084-7529

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Spatial resolution and the sensitivity to detect a fluorophore are the two most important optical parameters that characterize a confocal microscope. However, these are rather difficult to estimate quantitatively. We show that fluorescence correlation spectroscopy (FCS) provides an easy and reliable