Increasing mutagenic efficiency of gamma-radiation in rice by irradiation of seeds at extremely low temperature
✍ Scribed by H. Nakai; M. Saito
- Book ID
- 104617719
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 542 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
✦ Synopsis
Dry seeds of rice, OryzasativaL., containing about 13 percent moisture, were exposed to "'Co gamma rays at extremely low temperature (-196°C). M, damage on survival rate, seedling height and seed set were found to become much smaller in the cooling treatment than in the not-cooled control. Chlorophyll and heading date mutation frequencies in the M, generation were not affected by the decreased M, damage caused by the cooling treatment, and the mutation frequencies could be significantly increased by applying higher doses combined with the cooling treatment. Frequency of sterile or semi-sterile mutants in the M, generation was found to be significantly lower in the cooling treatment than in the not-cooled control.
It is assumed from the experimental results that extremely low temperatures during irradiation could reduce the amount of physiological damage and gross chromosome aberrations. This results in an increased efficiency of producing minute deletions and/or point mutations.