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Increased UV-induced SCEs but normal repair of DNA damage in p53-deficient mouse cells

✍ Scribed by Kanji Ishizaki; Yosuke Ejima; Tsukasa Matsunaga; Ryujiro Hara; Ayako Sakamoto; Mituo Ikenaga; Yoji Ikawa; Shin-Ichi Aizawa

Publisher
John Wiley and Sons
Year
1994
Tongue
French
Weight
442 KB
Volume
58
Category
Article
ISSN
0020-7136

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✦ Synopsis

UV-induced sister chromatid exchanges (SCEs) in p53deficient mouse cells were studied to obtain more evidence regarding the involvement of p53 protein in the DNA repair pathway as a checkpoint protein. After 5 J/m2 UV irradiation, mutant-type homozygous cells for p53-deficiency showed the same number of SCEs as the heterozygous and wild-type homozygous cells. In the heterozygous and wild-type homozygous cells, no further increase of SCEs was observed after 10 J/mz UV irradiation. In contrast, in mutant-type homozygous cells about twice as many SCEs were induced by 10 J/m2 UV as by 5 J/m2 UV. In mutant-type homozygous cells, fractions of S-phase cells decreased just after 10 J/m2 UV irradiation, but recovered to higher than control levels within a short time, while in heterozygous and wild-type homozygous cells, the decrease in S-phase cells was prolonged by more than 6 hr and no increase above control levels was observed. Although no difference in UV sensitivity and repair of UV-induced DNA damage was found among the 3 genotypes, which were determined by the relative colony-forming ability after UV irradiation

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