Recombinant Chinese hamster ovary (rCHO) cells expressing a high level of chimeric antibody were obtained by cotransfection of heavy-and light-chain cDNA expression vectors into dihydrofolate reductasedeficient CHO cells and subsequent gene amplification in medium containing stepwise increments in m
Increased Thermal Stability of Site-Selectively Glycosylated Dihydrofolate Reductase
โ Scribed by Richard S. Swanwick; Alison M. Daines; Lai-Hock Tey; Sabine L. Flitsch; Rudolf K. Allemann
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 104 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1439-4227
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Recombinant Chinese hamster ovary (CHO) cells expressing a high-level of chimeric antibody against S surface antigen of hepatitis B virus were obtained by co-transfection of heavy and light chain cDNA expression vectors into dihydrofolate reductase (dhfr)-deficient CHO cells and subsequent gene ampl
Previously, the highest producing (HP) recombinant CHO subclones isolated at various methotrexate (MTX) levels showed different antibody production stability during long-term culture, although they were clonally derived from CS13 transformant. In this study, genetic basis for their difference in ant